UV VIS SCANNING SPECTROPHOTOMETER

1. Applications

UV–Vis spectrophotometers are widely used in:

• Quantitative analysis (Beer–Lambert Law)

• Pharmaceutical analysis & drug identification

• DNA/RNA & protein estimation

• Environmental testing (water quality)

• Food & beverage quality control

• Chemical kinetics & reaction monitoring

• Academic and industrial research

2. Compliance Standards

Typical compliance and regulatory standards:

• USP (United states Pharmacopeia)

• EP (European Pharmacopeia)

• JP (Japanese Pharmacopeia)

• ISO/IEC 17025 (Calibration standards)

• 21 CFR Part 11 (Data integrity, optional software)

Key performance parameters defined:

• Wavelength accuracy

• Photometric accuracy

• Repeatability

• Stray light & resolution

3. Product Description

A UV–Vis scanning spectrophotometer is an analytical instrument that:

• Measures absorbance or transmittance of light in UV (190–400 nm) and visible (400–700 nm) regions

• Uses light sources (Deuterium for UV, Tungsten/Halogen for visible)

• Scans across wavelengths to produce spectra of samples

• Determines concentration based on light absorption characteristics

4. Technical Specifications (Typical)

(Values based on standard tender/lab-grade instruments)

5. Apparatus is Supplied With

• Main spectrophotometer unit

• Quartz & glass cuvettes

• Dust cover

• Power cable

• User manual

• Software (if PC-controlled)

• Calibration certificate

6. Common Capacities Available

• Standard cuvette size: 1 cm path length

• Microvolume (1–2 µL for advanced models)

• Multi-cell holders (4/6/8 cells)

• Sample volumes:

  • Micro: 1–100 µL

  • Standard: 1–5 mL

7. Optional Accessories

• Auto sampler

• Temperature-controlled cell holder

• Sipper system (flow-through)

• Integrating sphere (for solids/powders)

• Printer & PC software

• Validation kits (IQ/OQ/PQ)

• Fiber optic probes

8. Dimensions & Weight (Approx.)

• Dimensions: 450 × 350 × 200 mm (varies by model)

Weight: 10 – 25 kg