Neminath Instruments Neminath Instruments

Neminath Instruments

UV VISIBLE SPECTROPHOTOMETER

A UV–Visible spectrophotometer is widely used for qualitative and quantitative analysis of substances based on light absorption.

UV VISIBLE SPECTROPHOTOMETER

1. Application

  • Determination of concentration using Beer–Lambert law

  • Analysis of DNA, RNA, proteins (260/280 nm)

  • Pharmaceutical analysis (drug assay, dissolution testing)

  • Food and beverage analysis (color, additives)

  • Environmental monitoring (pollutants, metal ions)

  • Chemical kinetics studies

  • Identification of organic compounds and chromophores

  • Material and nanotechnology analysis

2. Compliance Standard

UV–Vis spectrophotometers must comply with international and pharmacopeial standards such as:

  • USP (United states Pharmacopeia)

  • Ph. Eur. (European Pharmacopoeia)

  • GLP / GMP guidelines

  • ISO / ASTM standards

Performance parameters required:

  • Wavelength accuracy

  • Photometric accuracy

  • Stray light limits

  • Resolution and baseline stability

3. Product Description

A UV–Visible spectrophotometer is an analytical instrument that measures the absorbance or transmittance of UV and visible light (190–900 nm) by a sample.

Working principle:

  • Light passes through the sample

  • Some wavelengths are absorbed

  • Detector measures transmitted light

  • Absorbance is proportional to concentration

Main components:

  • Light source (Deuterium + Tungsten lamp)

  • Monochromator (prism/grating)

  • Sample holder (cuvette)

  • Detector (photodiode/PMT)

  • Display/software system

  • 4. Technical Specifications (Typical)

Parameter

Specification

Wavelength range

190 – 900 nm (up to 1100 nm in some models)

Optical system

Single beam / Double beam

Spectral bandwidth

0.5 – 5 nm

Wavelength accuracy

±0.1 to ±0.3 nm

Photometric range

-4 to +4 Abs / 0–100% T

Photometric accuracy

±0.002 – 0.006 Abs

Detector

Silicon photodiode / PMT

Light source

Deuterium (UV), Tungsten (Visible)

Stray light

0.02% T

Scanning speed

Up to 3000 nm/min

Interface

USB / PC controlled

Power supply

220V, 50 Hz

 

5. Apparatus is Supplied With

Typical supply includes:

  • Main UV–Vis spectrophotometer unit

  • Quartz cuvettes (10 mm path length)

  • Glass/plastic cuvettes

  • Dust cover

  • Power cable

  • Software + PC interface

  • User manual

  • Printer (optional in some systems)

  • UPS (in advanced setups)

6. Common Capacities Available

  • Single beam spectrophotometer

  • Double beam spectrophotometer

  • Microvolume spectrophotometer (for DNA/protein)

  • Scanning UV–Vis spectrophotometer

  • Portable/compact models

Sample capacity:

  • Standard cuvette: 1–3.5 ml

  • Multi-cell holder: 4–8 samples at once

7. Optional Accessories

  • Auto-sampler

  • Temperature-controlled cuvette holder (Peltier system)

  • Integrating sphere (solid sample analysis)

  • Flow cell accessories

  • Sipper system

  • Software upgrades for kinetics & biomethods

  • External printer & data storage devices

 

8. Dimensions & Weight (Approx.)

(Varies by model, typical laboratory unit)

  • Dimensions: ~ 800 × 600 × 450 mm

  • Weight: ~ 25 – 35 kg

  • Example: Packing size ~860 × 660 × 465 mm, weight ~35 kg

 

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